- A lot of the pioneering work on transcription was carried out in prokaryotes, most notably in the bacteria E. Coli.
- Prokaryotes have a single RNA polymerase.
- Eukaryotes have 3 different RNA polymerase.
- Transcription in eukaryotes is more complex.
- Eukaryotic RNA polymerase cannot initiate transcription by themselves, they need the help of a set of proteins called the basic transcription factors.
- The enzyme that carries out transcription is called RNA polymerase, it consist of 4 polypeptides.
- Transcription can be divided into 3 phases: initiation, elongation, and termination.